DNA methylation patterns change in the course of embryonal cell development and general cellular differentiation. Thus, the effect of factors that cause sustainable changes in a cell (e.g., in vitro differentiation) are reflected by concomitant changes of methylation patterns. We can measure these changes and – since the signals are very robust –the measurements can be performed in high throughput. For high throughput analysis Epiontis uses its MS-SNuPE technology optimized to 96 or 384 well plates. Methylation patterns are detected using capillary electrophoresis or microplate based fluorescent readout. This allows screening for factors and culture conditions that stimulate, direct or inhibit cell differentiation.
